Effects of‘bushen huoxue decoctionon’ FSH and its receptor mRNA expression in mice

Objective:To investigate the effects of 'bushen huoxue decoction',a Chinese compound formula,on follicle stimulating hormone(FSH)and its receptor(FSHR)mRNA expression in mice with immune premature ovarian failure(POF).Methods:The model of POF in female BALB/c mice was established by multiple sites subcutaneous injection of zona pellucida,and injection of normal saline as blank control group.It was set up different dosage 'bushen huoxue decoction' of low,middle and high group,with prednisone and Conjugated Estrogens as western medicine-treated control group.ZPAb,FSH and E2 in serum of mice were detected by competitive ELISA,and the mRNA of FSHR in ovaries of mice was detected by real time fluorescent quantitative RT-PCR technology.Results:The concentration of ZPAb in serum of model group was higher than that of blank control group,but the concentration of E2 in serum of model group was lower than that of blank control group.There was significant difference in concentration of ZPAb and E2 between model group and blank control group(P0.01).The concentration of E2 in serum of the three 'bushen huoxue decoction' groups was higher than that of model group.There was significant difference in concentration of E2 between the three 'bushen huoxue decoction' groups and model group(P0.001).There was no statistic difference in concentration of FSH between model group and blank control group(P0.05).The serum FSH levels of the three 'bushen huoxue decoction' groups significantly increased compared with that of model group,and the difference was significant(P0.05).The mRNA of FSHR in ovaries of model group was less than that of blank control group.There was no statistic difference in mRNA of FSHR in ovaries between model group and blank control group(P0.05).The mRNA of FSHR in ovaries of the three 'bushen huoxue decoction' group was higher than in model group,and the difference was significant(P0.05).The concentration of E2 in serum of mice was linearly correlated positively with the mRNA expression of FSHR in ovaries(R2=0.359,F=64.888,P0.001).Conclusion:'Bushen huoxue decoction' increases the quantity of estrogen secreted by granular cells in ovaries by raised ovaries response to FSH through which 'Bushen huoxue decoction' increases the quantity of FSH secreted by pituitary gland and the level of FSHR in granular cells of ovaries.The level of E2 secreted by granular cells is linearly correlated positively with mRNA of FSHR in ovaries.