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HPLC determination of astragaloside IV in Buyang Huanwu mixture

Xie He-bing, Fang Qian

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Abstract

Objective:To develop a method for the determination of astragaloside Ⅳ in Buyang Huanwu mixture. Methods:HPLC determination of derivatized astragaloside Ⅳ,column temperature was 25 ℃,in the HPLC analysis using the ODS reversed-phase column Supelco C_(18)(4.6 mm×250 mm,5 μm) was used,mobile phase was metha- nol-water-tetrahydrofuran (90:6:4,0.02% trietylamine contained) at the flow rate of 1.2 mL·min~(-1),and the content was detected at the UV wavelength of 230 nm.Results:The linear range of derivatized astragaloside Ⅳ was 1.1-70.4 μg·mL~(-1)(r=0.9999).The average recovery(n=6) was 95.63% with RSD of 2.4%.Conclusions: The method is accurate and delicacy,and the absorbance of derivatized astragaloside Ⅳ is high.It can be adopted for the determination of astragaloside Ⅳ in Buyang Huanwu mixture.

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What this paper is about

Objective:To develop a method for the determination of astragaloside Ⅳ in Buyang Huanwu mixture. Methods:HPLC determination of derivatized astragaloside Ⅳ,column temperature was 25 ℃,in the HPLC analysis using the ODS reversed-phase column Supelco C_(18)(4.6 mm×250 mm,5 μm) was used,mobile phase was metha- nol-water-tetrahydrofuran (90:6:4,0.02% trietylamine contained) at the flow rate of 1.2 mL·min~(-1),and the content was detected at the UV wavelength of 230 nm.Results:The linear range of derivatized astragaloside Ⅳ was 1.1-70.4 μg·mL~(-1)(r=0.9999).The average recovery(n=6) was 95.63% with RSD of 2.4%.Conclusions: The method is accurate and delicacy,and the absorbance of derivatized astragaloside Ⅳ is high.It can be adopted for the determination of astragaloside Ⅳ in Buyang Huanwu mixture.

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Available abstract

Objective:To develop a method for the determination of astragaloside Ⅳ in Buyang Huanwu mixture. Methods:HPLC determination of derivatized astragaloside Ⅳ,column temperature was 25 ℃,in the HPLC analysis using the ODS reversed-phase column Supelco C_(18)(4.6 mm×250 mm,5 μm) was used,mobile phase was metha- nol-water-tetrahydrofuran (90:6:4,0.02% trietylamine contained) at the flow rate of 1.2 mL·min~(-1),and the content was detected at the UV wavelength of 230 nm.Results:The linear range of derivatized astragaloside Ⅳ was 1.1-70.4 μg·mL~(-1)(r=0.9999).The average recovery(n=6) was 95.63% with RSD of 2.4%.Conclusions: The method is accurate and delicacy,and the absorbance of derivatized astragaloside Ⅳ is high.It can be adopted for the determination of astragaloside Ⅳ in Buyang Huanwu mixture.

Key concepts: Chemistry, Chromatography, Astragaloside, High-performance liquid chromatography, Absorbance

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