Anti-laryngeal carcinoma of adenovirus vector-mediated HSV-tk gene in vitro

Objective:To observe killing action of HSV tk/GCV system on laryngeal cancer cells.Methods:Hep 2 cells were transfected by adenovirus vector with LacZ gene,then stained with X gal for measuring the adenovirus transfected efficiency.The recombinant adenovirus carring HSV tk was constructed by homologous recombination techniques.Hep 2 cells survival and bystanding effect were observed after adding 10 mg/L GCV to transfected cells.Results:The more the multiplicity of infection (MOI),the more the adenovirus transfected efficiency to Hep 2 cells.100% transfected efficiency needed MOI 200. The killing action of HSV tk/GCV on Hep 2 cells.Depended on the amount of AdCMVtk.There was weak bystanding effect.Conclusion:Adenovirus mediated HSV tk/GCV can kill Hep 2 cells.