Adenovirus vector mediated HSV-tk gene killing action on Lewis lung cancer cells

Objective To study the killing action of adenovirus vector carrying HSV-tk(AdCMVtk) on Lewis cells.Methods The recombinant adenovirus carrying HSV-tk was constructed by homologous recombination techniques.The adenovirus transfected efficiency to Lewis cells was measured,the cells were transfected by adenovirus vector with LacZ gene,then stained with X-gal.The effect of AdCMVtk/GCV system on survival rate of Lewis cells was observed.Results The adenovirus transfected efficiency to Lewis cells was increased with the increasing of multiplicity of infection(MOI).100% transfected efficiency needed MOI 500.AdCMVtk/GCV system could kill Lewis cells.When the amount of adenovirus or the concentration of GCV was increased,the cell survival rate was decreased,but AdCMVtk or GCV alone could not kill Lewis cells.The killing action existed bystander effect,74% Lewis cells were died with only 20% Lewis cells transfected AdCMVtk.Conclusion AdCMVtk/GCV system is effective and safe on killing tumor cells.