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Killing effect of adenovirus mediated HSV tk/GCV system on glioma C6 cell

Xu Zai

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Abstract

AIM: To observe killing action of HSV tk/GCV system on glioma cells. METHODS: C6 cells were transfected by adenovirus with LacZ gene, then stained with X gal for measuring the adenovirus transfected efficiency. C6 cells survival and bystander effect were observed after adding 10 mg·L -1 GCV to transfected cells. RESULTS: The more the multiplicity of infection (MOI), the more the adenovirus transfected efficiency to C6 cells ( r=0.819, P 0.05). 100% transfected efficiency needed MOI 100. The killing action of HSV tk/GCV on C6 cells, depended on the amount of AdCMVtk ( r=0.870, P 0.05). There was high bystander effect. CONCLUSION: Adenovirus mediated HSV tk/GCV system has good killing effect on glioma C6 cells.

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What this paper is about

AIM: To observe killing action of HSV tk/GCV system on glioma cells. METHODS: C6 cells were transfected by adenovirus with LacZ gene, then stained with X gal for measuring the adenovirus transfected efficiency. C6 cells survival and bystander effect were observed after adding 10 mg·L -1 GCV to transfected cells. RESULTS: The more the multiplicity of infection (MOI), the more the adenovirus transfected efficiency to C6 cells ( r=0.819, P 0.05). 100% transfected efficiency needed MOI 100. The killing action of HSV tk/GCV on C6 cells, depended on the amount of AdCMVtk ( r=0.870, P 0.05). There was high bystander effect. CONCLUSION: Adenovirus mediated HSV tk/GCV system has good killing effect on glioma C6 cells.

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Available abstract

AIM: To observe killing action of HSV tk/GCV system on glioma cells. METHODS: C6 cells were transfected by adenovirus with LacZ gene, then stained with X gal for measuring the adenovirus transfected efficiency. C6 cells survival and bystander effect were observed after adding 10 mg·L -1 GCV to transfected cells. RESULTS: The more the multiplicity of infection (MOI), the more the adenovirus transfected efficiency to C6 cells ( r=0.819, P 0.05). 100% transfected efficiency needed MOI 100. The killing action of HSV tk/GCV on C6 cells, depended on the amount of AdCMVtk ( r=0.870, P 0.05). There was high bystander effect. CONCLUSION: Adenovirus mediated HSV tk/GCV system has good killing effect on glioma C6 cells.

Key concepts: Bystander effect, Transfection, Multiplicity of infection, Glioma, Genetic enhancement, Molecular biology, Adenoviridae, Cell culture

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