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2011•Acta Agriculturae Boreali-SinicaRequires access

Research on Biological Effects and Molecular Markers of Maize Lines Implanted with Low Energy N~+ Ion Beam

Meixia Wang

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Abstract

Three groups of dry seeds of maize inbred lines,including Zheng58,Chang7-2 and K12 were implanted with different doses of low energy N+ ion beam.We analyzed the dates of germination percentage,germinating energy and agronomic characters,and find that to the energy 30 kV,7×1016 N+/cm2 was the best implanted dose.At the mean time,the cluster analysis was done by molecular markers with 10 pairs of SSR primers.39 alleles were detected,2-5 alleles per primer,average 3.9.Zheng58 of the three inbred lines implanted with N+ ion beam was the most sensitive.According to the cluster analysis,the best implanted dose was 7×1016 N+/cm2 to the energy 30 kV.The results of the two methods were almost consistent.

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What this paper is about

Three groups of dry seeds of maize inbred lines,including Zheng58,Chang7-2 and K12 were implanted with different doses of low energy N+ ion beam.We analyzed the dates of germination percentage,germinating energy and agronomic characters,and find that to the energy 30 kV,7×1016 N+/cm2 was the best implanted dose.At the mean time,the cluster analysis was done by molecular markers with 10 pairs of SSR primers.39 alleles were detected,2-5 alleles per primer,average 3.9.Zheng58 of the three inbred lines implanted with N+ ion beam was the most sensitive.According to the cluster analysis,the best implanted dose was 7×1016 N+/cm2 to the energy 30 kV.The results of the two methods were almost consistent.

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Available abstract

Three groups of dry seeds of maize inbred lines,including Zheng58,Chang7-2 and K12 were implanted with different doses of low energy N+ ion beam.We analyzed the dates of germination percentage,germinating energy and agronomic characters,and find that to the energy 30 kV,7×1016 N+/cm2 was the best implanted dose.At the mean time,the cluster analysis was done by molecular markers with 10 pairs of SSR primers.39 alleles were detected,2-5 alleles per primer,average 3.9.Zheng58 of the three inbred lines implanted with N+ ion beam was the most sensitive.According to the cluster analysis,the best implanted dose was 7×1016 N+/cm2 to the energy 30 kV.The results of the two methods were almost consistent.

Key concepts: Germination, Inbred strain, Allele, Ion, Primer (cosmetics), Cluster (spacecraft), Low energy, Beam energy

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