DNA Template Preparation and the Establishment of AFLP Technique for Almond Cultivar

AFLP analysis needs high quality DNA templates.Almond leaves contain high amounts of polyphenolics and polysaccharides,and therefore,the extraction of genomic DNA has been very difficult.In this study,gemomic DNA was extracted from different cultivar of almond using 3×CTAB extraction buffer and then purified with phenol/chloroform.Restriction analysis and preamplification indicated the DNA samples were high quality and therefore,used in the AFLP analysis.Results showed that the E+3/M+3 primer combination produced very few bands on genomic DNA of different almond cultivar,not suitable for the AFLP analysis,and the E+3/M+3 a great number of polymorphic bands in the range of 100～600 bp.The establishment of AFLP technique in this work is significant for the analysis of the genetic relatedness of almond species.