2007•Biotechnology(Faisalabad)Requires access

A Citrus DNA Extraction Method for AFLP Analysis

Lie Deng

Abstract

Objective:The traditional Citrus DNA extraction method used for AFLP analysis to get a more effective and efficient one has been adjusted.Methods:The genomic DNA samples of the accessions(fresh and clean leaves)using modified method were extracted,then electrophoresed with 0.8% gelose,finally followed AFLP operation.Results:The more clear AFLP fingerprint has been got.The result showed the DNA extracted by the method reach the quality AFLP requested,besides it is more effective and efficient.

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Key concepts: Amplified fragment length polymorphism, genomic DNA, DNA, DNA extraction, Extraction (chemistry), Biology, DNA profiling, Chemistry