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Extraction of DNA and Establishment of Fluorescent-AFLP Techniques in Leaves of Apricot

Yuzhu Wang

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Abstract

The high quality DNA required for construction of AFLP analysis system was isolated with an improved method of CTAB from young leaves of apricot.A fluorescent-AFLP reaction system was constructed for genetic analysis of apricot by optimizing DNA restriction-ligase reaction,pre-amplification and selective amplification.The clear and stable amplification patterns could be obtained from 12 pairs primer combinations with good polymorphism,which were selected from 64 pairs AFLP primer combinations.These results might provide a method based on the fluorescent-AFLP system for the further study in the field of molecular marker in cultivar identification,genetic diversity and genetic relationship in apricot.

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The high quality DNA required for construction of AFLP analysis system was isolated with an improved method of CTAB from young leaves of apricot.A fluorescent-AFLP reaction system was constructed for genetic analysis of apricot by optimizing DNA restriction-ligase reaction,pre-amplification and selective amplification.The clear and stable amplification patterns could be obtained from 12 pairs primer combinations with good polymorphism,which were selected from 64 pairs AFLP primer combinations.These results might provide a method based on the fluorescent-AFLP system for the further study in the field of molecular marker in cultivar identification,genetic diversity and genetic relationship in apricot.

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Available abstract

The high quality DNA required for construction of AFLP analysis system was isolated with an improved method of CTAB from young leaves of apricot.A fluorescent-AFLP reaction system was constructed for genetic analysis of apricot by optimizing DNA restriction-ligase reaction,pre-amplification and selective amplification.The clear and stable amplification patterns could be obtained from 12 pairs primer combinations with good polymorphism,which were selected from 64 pairs AFLP primer combinations.These results might provide a method based on the fluorescent-AFLP system for the further study in the field of molecular marker in cultivar identification,genetic diversity and genetic relationship in apricot.

Key concepts: Amplified fragment length polymorphism, Genetic diversity, Primer (cosmetics), DNA extraction, Biology, DNA, DNA profiling, Restriction enzyme

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