Comparison of TP- ELISA and TPPA on Syphilis

Objective: To compare TP- ELISA with TPPA on the test of syphilis antibody for the improvement of the relevance ratio and the exclusion of false positives. Methods: 286 positive samples with the absorbance 1. 0 and the numerical value 4. 348 were selected with the method of TP- ELISA. Then a definite diagnosis was made with TPPA. Follow- up observations were carried out about the positive samples with the absorbance 0. 6 and the numerical value 2. 61 tested with TP- ELISA. Result: The positive coincidence rate for the test of syphilis antibody with TP- ELISA was 91. 6%. As for the cases with the absorbance 1. 0 and the numerical value 4. 348 tested with TP- ELISA for positive results,the lower the absorbance and the numerical value were,the lower the positive coincidence rate of TPPA was. Conclusion: When syphilis antibody is tested with ELISA,weakly positive samples with OD value at the critical value should be rechecked with TPPA,and follow- up observations for considering the clinical symptoms.