Combining three ways to avoid the false positive of screening Treponema pallidum

Objective To investigate a method for avoiding the false positive of screening syphilis by Treponema pallidum antibody enzyme linked immunosorbent assay(TP-ELISA) though combining TP-ELISA with toluidine red unheated serum test(TRUST) and Treponema pallidum panicle agglutination test(TPPA).Methods The clinical samples were screened syphilis by TP-ELISA first,then the positive samples were further tested by TRUST and TPPA.Results There were 114 positive samples tested by TP-ELISA.Among them the number of the same result tested by TPPA was 111.The positive coincidence rate was 97.36%.Among the 111 positive samples,27 positive samples tested by TRUST were also positive tested by TPPA.The positive coincidence rate was 100%.There were 94 positive samples tested by TPPA among the remaining 97 negative samples tested by TRUST.The number of negative samples was 3.The mismatch rate was 2.64%.Conclusions The false positive result tested syphilis by TP-ELISA exists objectively.The positive samples from TP-ELISA were tested by TRUST and then were ascertained by TPPA.The positive result from TPPA can be diagnosed that syphilis antibody was positive.The negative result from TPPA can be diagnosed that syphilis antibody was negative.