Consistency of immune turbidimetry and ELISA in detection of syphilis antibody:a comparative study

OBJECTIVE To evaluate the consistency of immune turbidimetry(3TP) and ELISA for detecting syphilis specific antibody.METHODS A total of 10985 samples of outpatients and inpatients were detected by using ELISA for syphilis antibody,and those positive samples were examined by 3TP and TPPA.RESULTS Of 10985 samples,ELISA positive samples were 200,and the positive coincidence rate of ELISA and 3TP,TPPA was 92.5%.No sample missed the detection,and 15 samples were false positive by TP-ELISA.The false negative rate and false positive rate of ELISA method were 0%,1.7% respectively.Of 200 samples,the positive coincidence rate of 3TP and TPPA was 98.4%.3 samples were undetected and 3 samples were false positive by 3TP,and the false negative rate and false positive rate were 1.6% and 0.03% respectively;the difference in the positive detection rate among the three methods was not statistically significant;the 200 positive samples were divided into the high,medium and low absorption group according to the original value of ELISA,and the three methods were statistically compared;the results showed that the positive coincidence rates of ELISA,3TP and TPPA were above 95%,there was no statistical significance in the result;however,in the low absorption group,the positive coincidence rates of ELISA and 3TP,ELISA and TPPA,3TP and TPPA were 70.7%,70.7%,and 87.8% respectively,the difference in the detection result was statistically significant(P0.05).CONCLUSION Both ELISA and 3TP have high sensitivity and specificity,especially as it is the middle and high absorbance values,3TP has higher specificity than does ELISA,and has good consistency with TPPA so that it is available for the screening of large quantities of syphilis and confirmatory test.