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Determination of paeonol in Gucining capsule by RP - HPLC

Tonghui Li

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Abstract

OBJECTIVE To establish an RF - HPLC method to determine the contents of paeonol in Gucining capsule. METHODS The samples were extracted and analytical column was Tianhe chromatography C18(200 mm×4.6 mm) .The mobile phase consisted of CH3OH -H2O(58:42) and the flow rate was 1.0 ml·min-1 .Paeonol was detected at UV 274 nm.RESULTS The linear relationship was obtained in the range of 0.403- 1 .210 μg( r = 0.9999), the average recovery was 97.067, and the RSD was 2.27% ( n = 9). CONCLUSION This method is simple.sensitive and reliable. It can be used for the determination of paeonol in the Gucining capsule.

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What this paper is about

OBJECTIVE To establish an RF - HPLC method to determine the contents of paeonol in Gucining capsule. METHODS The samples were extracted and analytical column was Tianhe chromatography C18(200 mm×4.6 mm) .The mobile phase consisted of CH3OH -H2O(58:42) and the flow rate was 1.0 ml·min-1 .Paeonol was detected at UV 274 nm.RESULTS The linear relationship was obtained in the range of 0.403- 1 .210 μg( r = 0.9999), the average recovery was 97.067, and the RSD was 2.27% ( n = 9). CONCLUSION This method is simple.sensitive and reliable. It can be used for the determination of paeonol in the Gucining capsule.

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Available abstract

OBJECTIVE To establish an RF - HPLC method to determine the contents of paeonol in Gucining capsule. METHODS The samples were extracted and analytical column was Tianhe chromatography C18(200 mm×4.6 mm) .The mobile phase consisted of CH3OH -H2O(58:42) and the flow rate was 1.0 ml·min-1 .Paeonol was detected at UV 274 nm.RESULTS The linear relationship was obtained in the range of 0.403- 1 .210 μg( r = 0.9999), the average recovery was 97.067, and the RSD was 2.27% ( n = 9). CONCLUSION This method is simple.sensitive and reliable. It can be used for the determination of paeonol in the Gucining capsule.

Key concepts: Paeonol, Chemistry, Capsule, Chromatography, High-performance liquid chromatography, Linear range, Detection limit, Botany

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