Spectrophotometric Characteristics of Complex of Proteins with CPAIII
Zheng Zhang
Abstract
Zheng Zhang
Abstract
In a pH2.0 buffer solution,protein forms a green complex with CAPⅢ.Its maximum absorption peak is 685 nm with molar absorptivity of 1.8×105 L·mol-1·cm-1.Beer's law is obeyed in a range from 6.6 to 66 mg/L.The proposed method is applied to determination of protein in serum and peanut samples.
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In a pH2.0 buffer solution,protein forms a green complex with CAPⅢ.Its maximum absorption peak is 685 nm with molar absorptivity of 1.8×105 L·mol-1·cm-1.Beer's law is obeyed in a range from 6.6 to 66 mg/L.The proposed method is applied to determination of protein in serum and peanut samples.
Key concepts: Molar absorptivity, Spectrophotometry, Absorption (acoustics), Chemistry, Analytical Chemistry (journal), Buffer solution, Chromatography, Materials science