Effect of Tangshenning on Nonenzymatic Advanced Glycation End Products of Nephridial Tissue in Early Diabetic Nephropathy Rats

To study the effect of Tangshenning on nonenzymatic advanced glycation end products(AGEs) of nephridial tissue in early diabetic nephropathy rats.Methods:32 male SD rats were randomly divided into normal control group(group A,n=8) and diabetic group(n=24).Group A with conventional diet for 4 weeks,was injected intraperitoneally with 30mg/kg of citrate buffer.Diabetic group with high fat diet for 4 weeks,was injected intraperitoneally with 30mg/kg of STZ.After 2 weeks,24hU-Alb was measured.According to 24hU-Alb,the 24 SD rats were randomly divided into model group(group B,n=8),Tangshenning group(group C,n=8) and aminoguanidine group(group D,n=8).After 8 weeks,the level of blood glucose,24hU-Alb and tissue AGEs were measured.Results:The levels of blood glucose,24hU-Alb and AGEs in rats of group B were obviously higher than those of group A(all P0.01).The levels of 24hU-Alb and AGEs of group C and D were obviously lower than those of group B(all P0.01),but higher than those of group A(all P0.05).The levels of blood glucose of group C was obviously lower than those of group B,but group D was not obviously change.Conclusion:Similar to aminoguanidine,Tangshenning has effect on inhibiting the action of proteinum non-enzymatic glycosylation reaction and decreasing blood glucose and 24hU-Alb.