Effect of treatment with panax notoginseng saponins and aminoguanidine on nonenzymatic glycosylation in kidney of diabetic rats

Objective To investigate the renoprotective effect of treatment with panax notoginseng saponins(PNS) and aminoguanidine(AG) on streptozotocin(STZ)-induced diabetic rats as well as the possible mechanism involved in.Methods The rats diabetic model was induced by STZ.The model rats were divided into four groups:a diabetic group without treatment(group D,n=12),a diabetic group treatment with PNS(group P,n=12),a diabetic group treatment with AG(group A,n=12),and a diabetic group treatment with PNS and AG(group P+A,n=12).And there was a normal control group(group N,n=12) as well in the experiment.Their serum and renal cortex advanced glycation end products(AGEs) contents were determined by the spectrum law of fluorescence in the 4th weekend and 8th weekend of treatment respectively.The mean glomerular area(MGA) and volume(MGV) were measured by image analysis system.The changes of the blood glucose levels,urine amount,urine albumen,the renal function,body weight(BW),kidney weight(KW) and KW/BW ratio were observed.Results (1) Increased BW was observed in group P+A compared with that group D(P0.05) and its KW,KW/BW ratio,urinary excretion of protein,creatinine clearance rate(CCr),the content of AGEs in serun and renal cortex,MGA and MGV were significantly lower than that of group D(P0.01 or P0.05).(2) Compared with group D,CCr,the content of AGEs in serun and renal cortex,MGA,MGV,KW/BW ratio(the end of 8 week)and urinary excretion of protein( the end of 4 week) in group P were significantly decreased (P0.01 or P0.05).(3)The model rats of group A had significantly lower Ccr,serun AGEs contents,MGA,MGV and renal cortex AGEs contents (the end of 8 week)than group D(P0.01 or P0.05).(4)The content of serun AGEs,MGA and MGV(the end of 8 week)was decreased significantly in group P+A compared with group P and group A(P0.01 or P0.05),its the content of AGEs in renal cortex was significantly lower than that of group P(the end of 4 week)and group A (P0.05 respectively),and decreased KW/BW ratio was observed in group P+A compared with that group A(P0.05).Conclusions PNS and AG both possesses renoprotective effect on STZ-induced diabetic rats,whose mechenism be associated with inhibiting the overproduction of AGEs in renal tissue.The two unite and treat,it is better at single medical herses role for kidney to protect.