Protective effects of total flavonoids of Bidens bipinnata Lon acute inflammation and possible mechanism

AIM: To investigate the protective effects of total flavones of Bidens bipinnata L(TFB) on acute inflammation and its possible mechanism.METHODS: Mice ear edema model was induced by dimethylbenzene,and ear edema degree was measured 30 min later.Adjuvant arthritis(AA) rats model was established through intradermal injected with freund's complete adjuvant,and paw swelling degree was observed.Serum TNF-α,IL-1,IL-6,IL-8,and IL-2 contents were detected through enzyme linked immunosorbent assay(ELISA) in both models.Histopathological changes of knee joint were measured through hematoxylin-eosin staining,and the protein expression of ASIC1a in cartilage tissue was tested through immunohistochemistry.RESULTS:Compared with that of the model group,the ear edema degree and serum TNF-α,IL-1,IL-6,and IL-8 contents in the TFB(100,200 mg/kg) groups were decreased.Compared with that of the AA model rats,the paw swelling degree and the protein expression of ASIC1a in cartilage tissue were lower in TFB(67,133 mg/kg) groups,while serum IL-2 contents were elevated.The histopathological changes in the knee joint of AA rats including the number of inflammatory cells could be ameliorated by TFB(33,67,133 mg/kg).Results of Person correlation test demonstrated that ASIC1a protein was positively correlated with serum TNF-α,IL-1 and IL-8 concentrations.CONCLUSION: TFB has protective effects on acute inflammation,the mechanism might be associated with inhibitting the release of inflammatory mediators.