Effect of Total Flavones of Bidens bipinnata on Lipopolysaccharide-induced Acute Lung Injury in Rats

Objective:To investigate anti-inflammatory and antioxidant function of total flavones of Bidens bipinnata L(TFB) on lipoplysaccharide(LPS)-induced acute lung injury(ALI) and the mechanism.Method: One hundred and twenty SD rats were randomly divided into six groups:control group(physiological saline,ip),model group,dexamethasone group(5 mg · kg-1,ip),TFB treatment group(100,150,200 mg · kg-1,ip),twenty rats in each group.The rat ALI model was induced by LPS,5 mg · kg-1,ip.Six hours after modeling,all rats were sacrificed,protein content,white blood cell in bronchoalveolar lavage fluid(BALF),expression of NF-κB p65,and level of tumor necrosis factor(TNF-α),interleukin-6(IL-6),interleukin-10(IL-10)superoxide dismutase(SOD),malondialdehyde(MDA) and the activity of myeloperoxidase(MPO) in lung were measured.Result: Compared with control group,content of WBC and the protein in BALF was higher(P0.05-P0.01),compared with model group,NF-κB p65 expression level was(38.46±4.56)%,(31.39±4.18)%(in TFB high and medium dose group,respectively),the content of MDA was(1.73±0.19),(1.46±0.15)nmol · mg-1,and the content of TNF-α was(259.33±37.48),(211.46±32.69)μg · L-1,and the content of IL-6 was(287.46±100.18),(223.55±93.49)ng · L-1,and the activitiy of MPO was(2.69±0.57),(2.43±0.38)U · g-1,respectevly.NF-κB p65 expression level and the content of MDA,TNF-α,IL-6 and the activities of MPO in lung were significantly lower(P0.05-P0.01).On the other hand,the level of IL-10 was(16.73±3.87),(17.28±3.58)μg · L-1,the content of SOD was(69.46±9.84),(73.24±8.39)U · mg-1,the content of IL-10 and SOD was increased significantly(P0.05-P0.01).Conclusion: TFB can reduce pulmonary vascular permeability of LPS induced acute lung injury,reduce the inflammatory exudation and oxidative stress damage.