Content determination of Moxifloxacin Hydrochloride and Sodium Chloride Injection by RP-HPLC

Objective:To establish the RP-HPLC method for the content determination of Moxifloxacin Hydrochloride and Sodium Chloride Injection.Methods:The chromatographic analysis was performed on the DIKMA Inertsil Ph column(4.6mm×250mm,5 μm).The mobile phase was phosphate buffer(dissolving and diluting potassium dihydrogen phosphate 1.0 g,tetrabutylammonium hydrogensulfate 0.5 g and phosphoric acid 3.4 g to 1 000 mL with water)-methanol(72:28).The detection wavelength was at 293 nm.The flow rate was 1.3 mL.min-1 and the temperature was 45 °C,with the injection volume of 10μL.Results:There was a good linear relationship within the range of 82.12-110.35 μg/mL(r=0.9992).The reproducibility and precise of method was good.Conclusion:This method is accurate and reliable,which can be used for the quality control of Moxifloxacin Hydrochloride and Sodium Chloride Injection.