2006•Jiangsu Pharmaceutical and Clinical ResearchRequires access
Studies on Identification for Glibenclamide、Gliclazide、Glimepiride Mixed in TangLeShu Capsules by HPLC-MS Method
Dan Li
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Abstract
OBJECTIVE To establish a method for the identification of glibenclamide、gliclazide、glimepiride which mixed in a Chinese patent medicine Tangleshu capsules.METHOD The separation was performed on a VP-ODS C_(18)(2.0 mm×250 mm) analytical column,with the mixture of methanol and 0.2% acetic acid(70∶30) as the mobile phase′.ESI-MS was used to determin the synthetic chemicals HPLC methods.RESULT The same peaks as glibenclamide、gliclazide、glimepiride are found in Tangleshu capsules.CONCLUSION The method is feasible,accurate,reliable and can be used for identifying glibenclamide、gliclazide、glimepiride mixed in Tangleshu capsules.