ScholarLens Back to search
2009•Shandong Nongye Daxue xuebaoRequires access

CLONING AND SEQUENCE ANALYSIS OF BOVINE IL-2 GENE

Xie Kun

Open publisher page 0 citations

Abstract

According to cattle IL-2(M13204)gene sequence published by GenBank,a pair of primer was designed by primer premier 5 software,then A 477bp DNA fragment was amplified by RT-PCR technology using total RNA template extracted from Chinese Holstein Cattle blood lymphocyte that stimulate 24 hour by 1 milligram per milliliter concentration ConA.Agarose gel electrophoresis showed that the DNA fragment is about 477bp,the nucleotide homology between cloning cow IL-2 and cattle IL-2 reported by GenBank was 99.4%.The IL-2 gene homology between cow and horse,pig,cat,dog,goats,sheep was respectively 31.8%,32.0,31.6,30. 3%,30.8%,31.0%.

About this research paper

What this paper is about

According to cattle IL-2(M13204)gene sequence published by GenBank,a pair of primer was designed by primer premier 5 software,then A 477bp DNA fragment was amplified by RT-PCR technology using total RNA template extracted from Chinese Holstein Cattle blood lymphocyte that stimulate 24 hour by 1 milligram per milliliter concentration ConA.Agarose gel electrophoresis showed that the DNA fragment is about 477bp,the nucleotide homology between cloning cow IL-2 and cattle IL-2 reported by GenBank was 99.4%.The IL-2 gene homology between cow and horse,pig,cat,dog,goats,sheep was respectively 31.8%,32.0,31.6,30. 3%,30.8%,31.0%.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

According to cattle IL-2(M13204)gene sequence published by GenBank,a pair of primer was designed by primer premier 5 software,then A 477bp DNA fragment was amplified by RT-PCR technology using total RNA template extracted from Chinese Holstein Cattle blood lymphocyte that stimulate 24 hour by 1 milligram per milliliter concentration ConA.Agarose gel electrophoresis showed that the DNA fragment is about 477bp,the nucleotide homology between cloning cow IL-2 and cattle IL-2 reported by GenBank was 99.4%.The IL-2 gene homology between cow and horse,pig,cat,dog,goats,sheep was respectively 31.8%,32.0,31.6,30. 3%,30.8%,31.0%.

Key concepts: GenBank, Biology, Agarose gel electrophoresis, Molecular biology, Homology (biology), Gene, Cloning (programming), Primer (cosmetics)

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
CLONING AND SEQUENCE ANALYSIS OF BOVINE IL-2 GENE — Research Paper | ScholarLens