Proliferation inhibition effect of docetaxel combined with cisplatin during the course of chemotherapy for osteosarcoma

[Objective]To study the proliferation inhibition effect of decetaxel combined with cisplatin on osteosarcoma cells,and the effect of the drugs during the course of chemotherapy for osteosarcoma.[Method]The effect of docetaxel and cisplatin alone or combined with osteosarcoma cell line HOS-8603 was examined by cell count,morphologic observation,flow cytometry(FCM).The animal model of human osteosarcoma was established in nude mice.They were given docetaxel or cisplatin alone and combined,and a blank control group was set up.Observations on the growth and pathologic markers of osteosarcoma were carried out and pathological examinations were made.[Result]Docetaxel and cisplatin either alone or combined inhibited significantlythe proliferation of osteosarcoma cells and induced obviously apoptosis,and the effect of the drags combined was more effective than that of the drugs alone,Growth inhibition ratio ≥59.34%(P0.05).Apoptosis ratio 18.3l%(P0.01).Docetaxel or cisplatin alone inhibited significantly the growth of osteosarcoma,the tumor inhibitory rates 84.16%,78.53%;the drugs combined has more significant effect,the tumor inhibitory rates 96.78%.[Conclusion]Docetaxel and cisplatin combined has more significant effect of the proliferation inhibition of osteosarcoma cells and the tumor inhibition of osteosarcoma than that of the drugs alone.