Suppression of Osteosarcoma Cells Growth by 3-aminobenzamide Combined with Cisplatin

Objective To investigate the anticancer effects of cisplatin co-treated with PARP-1 inhibitor 3-aminobenzamide(3-AB) on human osteosarcoma cells. Methods Cell count kit-8 was used to analyze cell proliferation.In CCK-8 assay,U2OS cells were divided into two groups,one group was treated with cisplatin,and the other was treated with cisplatin plus 3-AB.Cell apoptosis and cell cycle were assessed by flow cytometric detection. Results The IC50 of U2OS cells by cisplatin plus 3-AB was lower than that by cisplatin alone,and the sensitized ratio was 1.76.It was showed that the apoptosis rate was increased in cells treated with 3-AB and cisplatin compared with that treated with two agents alone.And the combination treatment induced a more sustained S phase arrest. Conclusion PARP-1 inhibitor 3-AB enhances the anti-proliferation and apoptosis-inducing effects of cisplatin in human osteosarcoma cells.