Combined Somatostatin and IL-10 Treatment Inhibiting the Inflammatory Cytokines in Severe Pancreatitis Rat Model

Objective To investigate the effects of interleukin 10( IL-10) combined with somatostatin treatment on theinflammatory factors in severe acute pancreatitis( SAP) rat model.Methods Twenty-seven SD rats were randomly divided into three groups: group A was control group( n = 6),group B was SAP group( n = 6),group C was somatostatin intramuscular injection in combination with IL-10 intramuscular injection treatment group( n = 15).Group B was made into SAP models by retrograde cholangiopancreatography injection method.Group C was immediately injected with IL-10 and somatostatin.Serum were collected from the groups 1 hour,6 hours,12 hours after treatment,ELISA assay of serum inflammatory factors( TNF-α,L-6,IL-8) were performed.Results Increase of IL-6,IL-8,TNF-αlevels at 6 h,12 h were observed in both group B and group C,and a rising trend was observed,the difference was statistically significant( P 0.05),but IL-6,IL-8,TNF-α level of group C were lower than group B,the difference was statistically significant( P 0.05).Conclusion In SAP model,the application of IL-10 combined with somatostatin,could significantly inhibit serum levels of IL-6,IL-8,TNF-α,which is one of the treatments with perspectives for the clinical application.