Treatment role of Dahuangfuzi decotion on severe pancreatitis-acute lung injury

Objective To investigate the effects of Dahuangfuzi Decotion on serum proinflammatory and anti-inflammatory factors in severe acute pancreatitis and lung injury. Methods Thirteen health Landrace were randomly divided into three groups: control group( n = 4) ,model group( n = 4) ,treatment group ( n = 5) . The pigs model of SAP-ALI were induced by injecting 1 ml/kg of 4% sodium taurocholate into the main pancreatic duct. Serum levels of amylase,lipopolysaccharide( LPS) and TNF-α,IL-1β and IL- 4 by ELISA at once and in 6,12,24,48,72 h after the operation,the wet-to-dry( W/D) ratio of lung tissues, and pathomorphological changes of pancreatitis and lung tissue here observed. Results Serum concentration of amylase,LPS,TNF-α,IL-1β and pathologic scores of pancreas and lung in model group were significantly higher than those of the control group 6-72 h after sodium taurocholate induction( P 0. 05) . The serum concentration of interleukin-4 in model group were also higher than that of control group 24 h after operation( P 0. 05) . In comparison with model group,serum concentration of amylase,LPS,TNF-α,IL- 1β were significantly decreased in treatment group,but interleukin-4 increased beginning with 12 h after operation,reached the peak at 48 h( P 0. 05) . Conclusions Proinflammatory factor ( TNF-α and IL-1β) , anti-inflammatory factor ( IL-4 ) and LPS participate together in pathogenesis of SAP. Serum levels ofTNF-α,IL-1β were involved in the pathogenesis of SAP-ALI. Dahuangfuzi decoction can significantly reduce the degree of SAP-ALI through inhibiting the elevation of LPS and down-regulating expression of TNF-α,IL- 1β and up-regulating expression of IL-4.