Determination of Content of Quercetin and Kaempferol in Compound Ganyan Granules by HPLC

Objective: To establish a HPLC method for quantification of quercetin and kaempferol in compound Ganyan granules. Method: Samples were extracted in 80% methanol with ultrasonic process and hydrolyzed for 1 h. The HPLC was performed on an ODS-C 18 column(4. 6 mm × 250 mm,5 μm) with methanol-0. 4% H 3 PO 4(50∶ 50) as mobile phase. The flow rate was 1. 0 mL·min- 1and the column tempreture was kept at 30 ℃. The eluate was detected at 360 nm. Result: The linear ranges of quercetin and kaempferol were 0. 050 1-0. 501 μg(r = 0. 999 9) and 0. 024 2-0. 242 μg(r = 0. 999 5) respectively. The average recoveries of quercetin and kaempferol were 99. 69%(RSD 0. 22%) and 99. 30%(RSD 0. 82%). Conclusion: The method can be used for quality control of Ganyan granules. It is simple,rapid and accurate for simultancous determination of two constituents in compound Ganyan granules.