Morphological observation of A_(549) human lung cancer cells by the Raw Grub extract

Objective To observe the morphological changes of the apoptosis and degeneration of A549 human lung cancer cells in vitro after treatment with the raw extract of Grub.Methods The growth inhibition rates and cytotoxic effects of A549 cells were measured with MTT assay.Morphology changes were observed by microscope,HE and scan electron microscope.Results The Raw Grub extract could significantly inhibit the growth and proliferation of A549 cells in dose-dependent and time-dependent manner.Inverted microscope revealed that the A549 cells showed the apoptosis changes after treatedment with 80 μg/ml of the Grub petroleum ether extract before 24 h and necrosised;after 72 h.Conclusion The Grub extract has an influence on apoptosis induction and prdiferation inhibition of A549 human lung cancer cells in vitro.