STUDY ON EFFECT OF GRUB EXTRACT ON APOPTOSIS RELATIVE-GENEOF MGC-803 HUMAN GASTRIC CANCER CELL LINE IN VITRO

Objective:To discuss the mechanism of inducing apoptosis of grub extract on MGC-803 gastric cancer cell line in vitro.Methods:Using invert microscope,HE stain and acridine orange(AO)/ethidium bromide(EB)fluorescence stain to examine the inhibiting proliferation and inducing apoptosis effct of grub extract on MGC-803 gastric cancer cells.The expression changes of relating-apoptosis gene proteins(bcl-2,bax) were detected by immunohistochemistry(S-P method) before and after treaed with the grub extract.Results:The grub extract significantly inhibited the proliferation of gastric cancer cells in vitro,morphologic changes of apoptosis with chromatin margination,karyopyknosis,karyorrhexis,apoptotic body were found by the light scope.It was showed by using acridine orange(AO)/ethidium bromide (EB) fluorescence staining that MGC-803 gastric cancer cells were not only necrosis but also apoptosis after treated by the grub extract,but the apoptosis was as the dominant way.The bcl-2 and bax of relating-apoptosis genes was significantly changed before and after treated with the grub extract( P 0 001).Conclusions:The grub extract can significantly inhibit the proliferation and induce apoptosis of MGC-803 gastric cancer cells in vitro and its possible mechanism of inducing apoptosis for tumor cells might be related with the expression changes of the bcl-2 and bax of relating-apoptosis genes.