Determining indoor reference value of titer of anti-A/B IgG antibody in the pregnant women's serum using microcolumn gel assay

Objective To compare the differences between microcolumn gel assay and traditional test tube method in determining the titer of anti-A/B IgG antibody in the pregnant women's serum,to evaluate the sensitivity of microcolumn gel assay,and get the laboratory 's indoor reference value of microcolumn gel assay.Methods Using microcolumn gel assay and traditional test tube method to determine titer of serum anti-A/B IgG in 3 782 pregnant women then analyzing the results.Results The geometric mean of anti-A/B IgG antibody titers in pregnant women were 92.79,30.65 through microcolumn gel assay and traditional test tube method,the former was 3.03 times of the latter;the positive rate of microcolumn gel assay in determining the anti-A/B IgG antibody was obviously higher than that of traditional test tube method;as the indoor reference value,there was the same meaning between microcolumn gel assay of 1:256 and the traditional test tube method of 1:64.Conclusions The indoor reference value of microcolumn gel assay in determining the titer of anti-A/B IgG antibody in the pregnant women's serum should be set to 1:256;it's obviously that microcolumn gel assay has higher sensitivity and more advantages than traditional test tube method.