An Experimental Study on Inhibitory Effect of Artemeter on Colorectal Cancer Angiogenesis in BALB/c Mice

[Purpose]To explore inhibitory effect of Artmeter on colorectal cancer angiogenesis in mice. [Methods] Forty BALB/c mice (20 male and 20 female) with subcutaneous plantation of CT-26 colorectal cells (2×106) were randomly divided into 5 groups, 8 mice in each. They were low dose group (33.3mg/kg), middle dose group (50mg/kg), middle dose plus ferralia (50mg/kg+1.5mg/kg), high dose group (66.6mg/kg) and blank control group with normal saline. Treatment with stomach instillation started from day 1 to day 15. The mice were sacrificed at 24 hours after last treatment. The mice were weighted in Length-path (a mm) and short-path (b mm) of tumor in each mouse was measured by staff gauge every 3 days. Volume of tumor was calculated with Steel’s formula: V (mm3) =0.5ab2. Micro-vascular dense (MVD) was observed and countered under the microscopy by immunohistory chemistry. [Results] Micro-vascular count value in groups with high, middle, low and middle dose plus ferralia and control was 13±6,31±4,38±5 and 11±9, and 49±9 respectively. MVD in treatment groups was significantly lower than that in control group (P0.05,P0.01 respectively). [Conclusion] Artmeter at certain range of dosage has a significantly inhibitory effects on colorectal cancer angiogenesis in BALB/c mice.