2011•Journal of Southwest China Normal UniversityRequires access

Optimization of the Fermentation Conditions of the Endophytic Fungus Hd3

Min Zhang

Abstract

The components of liquid medium for strain Hd3 were studied by Plackett-Burman design and response surface methodology and their fermentation conditions including culture temperature,culture period,the pH value of medium,inoculate concentration and the volume of liquid medium of strains were optimized.The studied results of strain Hd3 showed that the suitable liquid medium was composed of 24.71 g/L glucose,1.08 g/L MgSO 4·7H 2O,244.17 g/L potato and distilled water;and the suitable cultivated condition was 31 ℃,9 d,9×103 cfu/mL and 90mL liquid medium in 250 mL flask,pH 6~7.

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Key concepts: Liquid medium, Fermentation, Strain (injury), Distilled water, Food science, Volume (thermodynamics), Chemistry, Chromatography