Optimizing the fermentation conditions of endophytic fungus BS002 of Sophora flavescens by response surface methodology

The endophytic fungus BS002 with a good disease-resistant effect was isolated from medicinal Sophora flavescens,and its fermentation medium conditions were studied.Based on the monofactorial experiments and the Plackett-Burman design test it was concluded that the crystalline sodium acetate,potato and glucose contents were main influencing factors! The fermentation conditions of endophytic fungus BS002 were optimized by response surface methodology and the BoxBehnken design test,and the gained optimal conditions were as follows:100 mL solution in a 250-mL conical flask,246.47 g/L potato,27.81 g/L glucose,1.95 g/L crystalline sodium acetate,10~7 cfu/mL pitching rate,and 1.0 g/L yeast extract paste at 25 ℃ and 150 r/min speed for 4 d,in which the mycelium dry weight could reach 10.398 g/L.