Effects of Matrine on Proliferation,Apoptosis and Survivin Gene Expression in Human Cervical Cancer Hela Cells

Objective: To investigate the effects of matrine on proliferation,apoptosis in human cervical cancer Hela cells and its possible molecular mechanism.Method: Hela cells were treated with matrine of different concentrations.MTT assay was used to measure the cell proliferative effect.The Annexin V FITC / PI double staining analysis by flow cytometry was used to evaluate apoptotic rate and the cell cycle.RT-PCR and western blotting were performed to detect the expression of Survivin gene.Result: Matrine could inhibit the proliferation of Hela cells in vitro,and dose-effect relationship was significant(P 0.01).IC50 of Hela cells was 2.60 g.L-1.The flow cytometry test indicated that matrine could make Hela cells G0-G1 phase gradually increasing,G2-M period and S phase reduce gradually,and with the increase of the dose,Hela cells apoptosis rate increased significantly(P 0.01).Matrine could suppress the expression of Survivin gene(P 0.01),and with the dose-dependent manner.Conclusion: Matrine could effectively inhibit proliferation and induce apoptosis of Hela cells.Its molecular mechanisms might be related to modulating the expression of Survivin.