The Significance of Nucleostemin Gene and PCNA in Non-small Cell Lung Cancer Detection

Objective:To investigate the detection and significance of Nucleostemin proteins and PCNA in non-small cell lung cancer(NSCLC)tissue.Method:The clinical studies were detected by immunohistochemistry SP method in 53 cases of NSCLC patients with non-small cell lung cancer and 15 cases of adjacent normal tissues NS protein and its clinical and pathological characteristics of patients with NSCLC were analyzed.Result:The NS protein expression in NSCLC was 54.7%(29/53),it was significantly higher than in adjacent normal tissue expression rate(0/15);Adenocarcinoma of the NS protein expression was 65.2%(15/23),it was significantly higher than the expression rate 46.7%(14/30)of the squamous cell carcinoma;The expression of differentiated tissues was 42.9%(15/35),it was significantly lower than the rate of poorly differentiated tissues 77.8%(14/18),there were statistically significant differences(P0.05).And it had no relationship with TNM stages and lymph node metastasis(P0.05).The positive rate of PCNA in NSCLC group was 71.7%(38/53),it was significantly higher than that in normal tissues6.7%(1/15),the difference was statistically significant between the groups(P0.05).Conclusion:NSCLC patients with non-small cell lung cancer,NS mRNA and protein high expression of trend is conducive to the malignant proliferation of tumor cells,the positive rate of PCNA is significantly higher,and thus is a high clinical value of tumor molecular markers.