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2013•Dalian Gongye Daxue xuebaoRequires access

Determination of total ginsenosides from the stems and leaves and ginsenoside Rb_3 in different extracts of Panax ginseng by RP-HPLC

Gong Xiao-jie

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Abstract

RP-HPLC method was developed to determine ginsenoside Rb3 in different extracts of Panax ginseng and total ginsenosides from the stems and leaves of Panax ginseng on Nava-Pak ODS column(3.9 mm×150 mm,4 μm).The mobile phase was V(methanol)∶V(water)∶V(phosphoric acid)=65∶35∶1,the detection wavelength was set at 203 nm,the flow rate was 1.0 mL/min.The content of ginsenoside Rb3 was determined with external standard method.The ginsenoside Rb3 had a good linearity from 0.8 to 20.0 μg(R=0.999 9),and the average recovery was 100.3%.

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What this paper is about

RP-HPLC method was developed to determine ginsenoside Rb3 in different extracts of Panax ginseng and total ginsenosides from the stems and leaves of Panax ginseng on Nava-Pak ODS column(3.9 mm×150 mm,4 μm).The mobile phase was V(methanol)∶V(water)∶V(phosphoric acid)=65∶35∶1,the detection wavelength was set at 203 nm,the flow rate was 1.0 mL/min.The content of ginsenoside Rb3 was determined with external standard method.The ginsenoside Rb3 had a good linearity from 0.8 to 20.0 μg(R=0.999 9),and the average recovery was 100.3%.

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Available abstract

RP-HPLC method was developed to determine ginsenoside Rb3 in different extracts of Panax ginseng and total ginsenosides from the stems and leaves of Panax ginseng on Nava-Pak ODS column(3.9 mm×150 mm,4 μm).The mobile phase was V(methanol)∶V(water)∶V(phosphoric acid)=65∶35∶1,the detection wavelength was set at 203 nm,the flow rate was 1.0 mL/min.The content of ginsenoside Rb3 was determined with external standard method.The ginsenoside Rb3 had a good linearity from 0.8 to 20.0 μg(R=0.999 9),and the average recovery was 100.3%.

Key concepts: Ginseng, Ginsenoside, Chemistry, Chromatography, High-performance liquid chromatography, Phosphoric acid, Araliaceae, Medicine

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Determination of total ginsenosides from the stems and leaves and ginsenoside Rb_3 in different extracts of Panax ginseng by RP-HPLC — Research Paper | ScholarLens