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Content Determination of Tanshinone IIA in Danqishujin Pills

Yuxin He

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Abstract

To establish a method for the content determination of Tanshinone ⅡA in Danqishujin Pills,HPLC was adopted with a Kromasil-C18(200mm×4.6mm,5μm)column.The mobile phase consisted of methanol-water(75:25) and the detecting wavelength was set at 270nm.The flow rate was 1.0ml·min-1 with column temperature of 35℃.The linear range of Tanshinone ⅡA was 0.020～0.200μg(r=0.9999),and the average recovery was 99.06% with RSD of 0.80%(n=6).The method is considered to be convenient and accurate with good reproducibility,so it is suitable to the content determination of tanshinone ⅡA in danqishujin pills.

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What this paper is about

To establish a method for the content determination of Tanshinone ⅡA in Danqishujin Pills,HPLC was adopted with a Kromasil-C18(200mm×4.6mm,5μm)column.The mobile phase consisted of methanol-water(75:25) and the detecting wavelength was set at 270nm.The flow rate was 1.0ml·min-1 with column temperature of 35℃.The linear range of Tanshinone ⅡA was 0.020～0.200μg(r=0.9999),and the average recovery was 99.06% with RSD of 0.80%(n=6).The method is considered to be convenient and accurate with good reproducibility,so it is suitable to the content determination of tanshinone ⅡA in danqishujin pills.

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Available abstract

To establish a method for the content determination of Tanshinone ⅡA in Danqishujin Pills,HPLC was adopted with a Kromasil-C18(200mm×4.6mm,5μm)column.The mobile phase consisted of methanol-water(75:25) and the detecting wavelength was set at 270nm.The flow rate was 1.0ml·min-1 with column temperature of 35℃.The linear range of Tanshinone ⅡA was 0.020～0.200μg(r=0.9999),and the average recovery was 99.06% with RSD of 0.80%(n=6).The method is considered to be convenient and accurate with good reproducibility,so it is suitable to the content determination of tanshinone ⅡA in danqishujin pills.

Key concepts: Chromatography, Reproducibility, Linear range, Content determination, High-performance liquid chromatography, Chemistry, Analytical Chemistry (journal), Mathematics

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