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2012•Strait Pharmaceutical JournalRequires access

Determination of Tanshinone IIA in Jinsang Sanjie pills by RP-HPLC

Huan‐Huan Jiang

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Abstract

OBJECTIVE To establish an HPLC method for determining the content of Tanshinone ⅡA in Jinsang Sanjie pills.METHODS Shimadzu VP-ODS(150mm×4.6mm,5μm) was used,Acetonitrile-water(75∶25)was used as mobile phase.The detection wavelength was 270nm and the flow rate was 1.0mL·min-1.External standard method was used.RESULTS TanshinoneⅡA concentration presented a good linear range of 0.37μg·mL-1～36.74μg·mL-1(r=0.9999),the average recovery was 99.9%,RSD=1.7%.CONCLUSION The method is simple,rapid and accurate for quality control of the preparation.

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What this paper is about

OBJECTIVE To establish an HPLC method for determining the content of Tanshinone ⅡA in Jinsang Sanjie pills.METHODS Shimadzu VP-ODS(150mm×4.6mm,5μm) was used,Acetonitrile-water(75∶25)was used as mobile phase.The detection wavelength was 270nm and the flow rate was 1.0mL·min-1.External standard method was used.RESULTS TanshinoneⅡA concentration presented a good linear range of 0.37μg·mL-1～36.74μg·mL-1(r=0.9999),the average recovery was 99.9%,RSD=1.7%.CONCLUSION The method is simple,rapid and accurate for quality control of the preparation.

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Available abstract

OBJECTIVE To establish an HPLC method for determining the content of Tanshinone ⅡA in Jinsang Sanjie pills.METHODS Shimadzu VP-ODS(150mm×4.6mm,5μm) was used,Acetonitrile-water(75∶25)was used as mobile phase.The detection wavelength was 270nm and the flow rate was 1.0mL·min-1.External standard method was used.RESULTS TanshinoneⅡA concentration presented a good linear range of 0.37μg·mL-1～36.74μg·mL-1(r=0.9999),the average recovery was 99.9%,RSD=1.7%.CONCLUSION The method is simple,rapid and accurate for quality control of the preparation.

Key concepts: Chromatography, Chemistry, High-performance liquid chromatography, Linear range, Content determination, Analytical Chemistry (journal), Detection limit

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