RESEARCH ON AFFECTIVE FACTOR OF SOYBEAN CALLUS INDUCTION

Among the 3 media tested,MS was the most suitable for the callus induction of soybean.The medium added hormone with 0.5mg/L 6-BA and 2.0 mg/L 2,4-D was the best one for the induction of callus.Neither higher nor lower would suitable for the induction of callus.The effects of explants type on soybean callus induction were different,and the hypocotyls was more effecive than cotyledon on soybean callus induction.Among the 6 soybean types,zhonghuang13 was the most suitable for the callus induction of soybean.12 hour light and 12 hour dark were benefit for the callus induction.The best pH for the callus induction and subculture was between 5.8 and 6.0.