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HPLC Determination and Comparison of Vitamin C Content in Three Fruits

Mi Tang, Zhao Jian-ming, Y Zhang, YU Ju-hong, Duan Ling-li, Luo Ya-jun, Bian Qing-quan

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Abstract

Establish HPLC analysis method of vitamin C of tomatoes,kiwi,jujube,and analyze comparatively vitamin C content of them. Shimadzu LC-20 A HPLC system for quantitative analysis of the sample,the chromatographic column of ODS; 0.1% oxalic acid solution as the mobile phase; detection wavelength of 240nm; temperature of column was 35℃; flow rate of 1.2mL·min-1. Under these conditions,the target component and other components were separated effectively(R1.5),the sample volume in the range of 0.144-1.44μg present good linear correlation,coefficient r=0.9998,average recovery rate: 99.1%,RSD: 0.3%. This method is of specific property,good separation,fast analysis speed,high sensitivity,simple operation,accurate and reliable result. The results can provide a reference for the depth of exploration of kiwi,tomatoes and jujube.

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What this paper is about

Establish HPLC analysis method of vitamin C of tomatoes,kiwi,jujube,and analyze comparatively vitamin C content of them. Shimadzu LC-20 A HPLC system for quantitative analysis of the sample,the chromatographic column of ODS; 0.1% oxalic acid solution as the mobile phase; detection wavelength of 240nm; temperature of column was 35℃; flow rate of 1.2mL·min-1. Under these conditions,the target component and other components were separated effectively(R1.5),the sample volume in the range of 0.144-1.44μg present good linear correlation,coefficient r=0.9998,average recovery rate: 99.1%,RSD: 0.3%. This method is of specific property,good separation,fast analysis speed,high sensitivity,simple operation,accurate and reliable result. The results can provide a reference for the depth of exploration of kiwi,tomatoes and jujube.

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Available abstract

Establish HPLC analysis method of vitamin C of tomatoes,kiwi,jujube,and analyze comparatively vitamin C content of them. Shimadzu LC-20 A HPLC system for quantitative analysis of the sample,the chromatographic column of ODS; 0.1% oxalic acid solution as the mobile phase; detection wavelength of 240nm; temperature of column was 35℃; flow rate of 1.2mL·min-1. Under these conditions,the target component and other components were separated effectively(R1.5),the sample volume in the range of 0.144-1.44μg present good linear correlation,coefficient r=0.9998,average recovery rate: 99.1%,RSD: 0.3%. This method is of specific property,good separation,fast analysis speed,high sensitivity,simple operation,accurate and reliable result. The results can provide a reference for the depth of exploration of kiwi,tomatoes and jujube.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Oxalic acid, Content determination, Correlation coefficient, Linear relationship, Linear correlation

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