2007•Journal of Hengyang Normal UniversityRequires access

Study on the Content of Vitamin C in the Tomato by HPLC

Jie Bai

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Abstract

In this article,HPLC was performed to research the content of Vitamin C in the tomato.In this way,the content,the repeatability and the returns-ratio of the Vitamin C in the tomato were analyzed under the condition of that ultra-pure water was taken as flowing,and its speed was 1.0ml/min,and the ultraviolet examination wave length was 267nm with the column temperature under the 25℃.The good linear relations were gotten through the linear return with its correlation coefficient being R2=0.9994.Its returns-ratio was 98.71%~103.29%.The relative standard deviation was 0.79%.The method established was simple,fast and accurate,which will be suitable to analyze the content of the Vitamin C in the tomato.

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What this paper is about

In this article,HPLC was performed to research the content of Vitamin C in the tomato.In this way,the content,the repeatability and the returns-ratio of the Vitamin C in the tomato were analyzed under the condition of that ultra-pure water was taken as flowing,and its speed was 1.0ml/min,and the ultraviolet examination wave length was 267nm with the column temperature under the 25℃.The good linear relations were gotten through the linear return with its correlation coefficient being R2=0.9994.Its returns-ratio was 98.71%~103.29%.The relative standard deviation was 0.79%.The method established was simple,fast and accurate,which will be suitable to analyze the content of the Vitamin C in the tomato.

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Available abstract

In this article,HPLC was performed to research the content of Vitamin C in the tomato.In this way,the content,the repeatability and the returns-ratio of the Vitamin C in the tomato were analyzed under the condition of that ultra-pure water was taken as flowing,and its speed was 1.0ml/min,and the ultraviolet examination wave length was 267nm with the column temperature under the 25℃.The good linear relations were gotten through the linear return with its correlation coefficient being R2=0.9994.Its returns-ratio was 98.71%~103.29%.The relative standard deviation was 0.79%.The method established was simple,fast and accurate,which will be suitable to analyze the content of the Vitamin C in the tomato.

Key concepts: Repeatability, Relative standard deviation, High-performance liquid chromatography, Linear relationship, Content (measure theory), Correlation coefficient, Chromatography, Linear correlation

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