2006•Zhongguo shouyi zazhiRequires access
Preparation and identification of the monoclonal antibody specific to hemagglutinin of subtype H9 avian influenza virus
Qiang Jia
Open publisher page 0 citations
Abstract
Two strains of hybridoma cultures secreted monoclonal antibody specific to hemagglutin of subtype H9 avian influenza viruses were developed by fusing the spleen cell of Balb/c mice which immunized with subtype H9 avian influenza viruses and SP2/0 myeloma cell.Identified by HI and Western blot,they were.monoclonal antibodies specific to the protein HA1 of subtype H9 avian influenza viruses.The ELISA titers of ascites were 1∶10~7 and 1∶10~6;the HI titers of both ascites were 15 log_2.