Development and application of monoclonal antibody specific to hemagglutinin of H9 subtype avian influenza viruses

Objective:To development monoclonal antibody ( McAb) specific to hemagglutinin of H9 subtype avian influenza viruses( AⅣ). Methods: Fusing SP2/ O-Ag-14 myeloma cells with spleen cells of BALB/ c mice immunized with live AⅣ-H9 subtype vaccine, and positive hybridoma clones were screened by HI test with supernatant. The specificity of McAbs was characterised by HI test ,ELISA and Western blot. Results:Nine stains McAb against HA of AIV H9 subtype viruses were obtained. All of these only specifically reaction with most of 82 strains of subtype AⅣ-H9 viruses, which isolated in chicken and duck in different provinces in different year, but not with other viruses. Specificity analysis by Western blot confirm that eight of these McAbs recognize HA, and one of the nine McAbs reaction with all of the detected H9 subtype viruses. Conclusion: Nine McAbs specific to hemagglutinin of AⅣ-H9 were obtained, which provided useful tool for virus antigenic epitope analysis .clinical diagnosis and epidemiological investigation.