Study on the preparation and influencing factors on the particle size of gelatin microspheres

Gelatin microsphere(GMS) was prepared through emulsion chemical-crossline method. The pre-paration conditions of GMS such as stirring speed,emulsifier dosage,gelatin concentration,ratio of water to oil phase and emulsion time were studied and discussed. Orthogonal experiment was made to study the influence of stirring speed,gelatin concentration and water/oil rate. The result shows that enhance stirring speed,decrease gelatin concentration or water/oil rate benefit to reduce the particle size of GMS and increase it's sphericity. Optimization experiment shows that the excellent result was obtained with the condition:0.20 g/mL of emulsifier dosage,800 r/min stirring speed,1∶6 of water/oil rate,1.5% of emulsifier dosage and 15 min of emulsifying time. The GMS prepared at this condition had an average particle size as 11.6 μm and the best sphericity dispersion.