2004•Zhonghua wuli yixue zazhiRequires access
The effects of electromagnetic pulse on activity of N-methyl-D-aspartate receptor in hippocampus of rats
Yuhong Li, Jin Li, Su Ruibin AWu Ning, Li Zijian AWang Shuiing, Rui-yuan Peng
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Abstract
Objective To investigate the relationship between the changes of N-methyl-D-aspartate(NMDA) receptor activity in hippocampus of rats and electromagnetic pulses(EMP) exposure. Methods Thirty-two rats were divided into a control group (6 rats) and an experimental group (26 rats),subject to EMP irradiation. The rats in the experimental group were decapitated after EMP(6×10 4V/m,rise time 20ns,pulse width 30μs,5 pulses in 2 minutes) irradiation and hippocampus were sampled,and NMDA receptor activity were detected with a radioligand binding assay with 3H-Glu. The rats in the control group were treated the same except EMP exposure. Results The Kd value of NMDA receptor decreased significantly at 3h after irradiation,then increased gradually after 6h and reached normal level at 48h after irradiation. The Bmax value of the receptor decreased significantly at 3～6h after exposure,then began to rise at 24h and reached a higher level than that of the control group. Conclusion EMP irradiation induced the decrease of both Kd and Bmax values of NMDA receptors in hippocampus of rats. It was suggested that the changes of density and affinity of NMDA receptor might be partly responsible for the cognitive deficits of rats.