The initial research on the effect of anti-stressed depression of Jiaweisinisan and it's mechanism of N-methyl-D-aspartate receptor channel in hippocampus

Aim To study the anti-stress depression effect of Jiaweisinisan(JWSNS)and it's mechanism of N-methyl-D-aspartate(NMDA)receptor channel in hippocampus.Methods Chronic mild unpredictable stress(CMUS)was adopted to establish the rat depres-sion model.Before and after model establishing,the sucrose consumptions and the weight of rats were detected.TTC dyeing of hippocampus brain slices ex vivo were used to detect the situation of hippocampus neuron damage and the protective effect of serum containing components of JWSNS.The opening probability and the average opening time of NMDA receptor channels were detected by attach on cell patch clamp,the Ca2+ concentrations in single cell of hippocampus neurons were detected by Tillvis ION software.Results CMUS could decrease the sucrose preference and the weights of rats(P0.01),JWSNS and MK801 could increase sucrose preference,but had no effects on the weights.The formula of JWSNS,the 20% serum containing components of JWSNS and MK801 could significantly increase the OD values of hippocampus brain slices TTC staining and decrease the hippocampus neuron damage rates(P0.05,P0.01).The opening probability of NMDA receptor channels in model group was higher than that of normal group(P0.05),while JWSNS and MK801 could significantly decrease the opening probability of NMDA receptor channels in hippocampus(P0.05,P0.01).But there were no statistical significance among groups in the average opening time of NMDA receptor channels.Meanwhile,JWSNS and MK801 could obviously decrease the Ca2+ concentration in single cell of hippocampus neurons(P0.01).Conclusion JWSNS has the effect of anti-stress depression.It is suggested that NMDA receptor may be one of the pharmacological targets of JWSNS for anti-stress depression by directly regulating the function of NMDA receptor.