Researching the Inhibitory Effect of Xuanfu Guilian Prescription on Eca9706 Esophagus Carcinoma Cell Proliferation from the Cell Cycle and Apoptosis

Objective: From the cell proliferation,cycle and apoptosis researching the mechanism which Xuanfu Guilian prescription treats esophageal cancer.Method: Esophageal carcinoma cells(Eac9706) in vitro were cultured,and the cell proliferation inhibitory rate of Xuanfu Guilian prescription were detected with MTT assay to observe the time-and dose-effect;1 × 105cells / wells were seeded in 6 wells plate and respectively mixed in Xuanfu Guilian prescription extracts(15,25,60 mg·L-1);after cultured 48 h,the cell cycle and apoptosis of Eac9706 were detected by flow cytometry(FCM).Result: Xuanfu Guilian prescription can inhibit strongly the proliferation of Eca9706 cell,and have dose dependent,50% inhibition concentration(IC 50) 58.11 mg·L-1.The inhibition rate of concentration 15,25,60 mg·L-1increased on time in 96 h.Early and late apoptosis rate of treatment group were significantly increaser than the negative(P 0.05).The cells rate of G 0 / G 1 also increased significantly(P 0.05),and the 60 mg·L-1group appeared apoptosis peak.Conclusion: Xuanfu Guilian prescription can inhibit the proliferation of Eac9706 cells and there is in dose and time dependent,which mechanism may be related to drug block of cells to G 0 / G 1 phase and induce cell apoptosis.