Effects of Celecoxib on the Proliferation and Apoptosis of Human Esophageal Carcinoma Cell Line Eca-109

Objective To determine the effect of Celecoxib on the proliferation and human esophageal carcinoma cell Line Eca-109 and the probable mechanism involved.Methods Using Methabenzthiazuron(MTT) assay,flow cytometry(FCM),Acridine orange staining.The effects of celecoxib on the proliferation and apoptosis of Eca-109 cells as well as its related mechanism were studied.Results The growth of Eca-109 cells was inhibited apparently by celecoxib in a time and dose dependent manners.Sub-G1 peak was detected by FCM,and apoptotic rate of which was(15.89±0.87)%~(73.57±1.63)%.The cell ratio of G0/ G1 phase increased,whereas the cell ratio of S and G2/M phases decreased after treatment,which was in a dose-dependent manner as well.The treated Eca-109 cells exhibited some morphological features of apoptosis,including cell shrinkage,nuclear condensation,DNA fragmentation,and the formation of apoptosis bodies,the apoptotic index of which was in a time and dose dependent manners.Conclusions Celecoxib inhibited proliferation and induced apoptosis of Human Esophageal Carcinoma Cell Line Eca-109,which may be related to blocking the cell cycle progress of Eca-109 cells.