Comparison of blood cell analyzer and peripheral blood smear on the white cell classification results in patients with malignant hematological diseases

Objective To reduce the error of white blood cell classification,compensate the cell morphology identification ability,and improve the accuracy of peripheral white blood cell classification of the blood cell analyzer. Methods Samples of75 patients with hematological diseases were collected from the Tangshan Iron and Steel Hospital. After anticoagulated by EDTA-K2,samples were used for the detection of CBC+diff using the Sysmex XT-1800i whole blood five classification system,at the same time abnormal white blood cell classification specimen prepared two pieces of two blood smears were prepared from the same sample and stained with Wright-Giemsa staining and counted for different types of white blood cells such as neutrophils,lymphocytes and monocytes. Results By the comparison of determination of blood smear and blood cell counting instrument,differences mainly display in the counting of lymphocytes and monocytes by blood smear method and the blood counting instrument were significantly different(47.35±18.14)vs(38.40±17.60);(6.80±2.12)vs(12.68±1.40)(t=12.650,P 0.05;t =15.200,P 0.05). Conclusion The discrepancy generated in the blood counting instrument might be due to the chromosome abnormalities in the patients white blood cells,resulting in the over estimate of monocytes and under estimate of lymphocytes,therefore compensate by blood smear examination is important.