Diagnostic significance of combined detection of serum anti cyclic citrullinated-peptide antibody and rheumatoid factor in rheumatoid arthritis

Objective To evaluate the significance of the combination of serum anti cyclic citrullinated peptide antibody(anti CCP)and rheumatoid factor(RF)in the diagnosis of rheumatoid arthritis(RA).Methods ELISA and IRN techniques were used to detect serum levels of anti CCP and RF in 59 patients with RA and 48 patients without RA.Results Positive anti CCP or RF alone was found in 8 or 16 patients with RA,respectively.while positive anti CCP and RF were found in 30 patients with RA.Positive anti CCP or RF alone was only noticed in two or 14 patients without RA.Both anti-CCP and RF positive were not observed in the patients without RA.The positive rate of anti CCP or RF alone and both of patients with RA was 64.40%,77.97%,and 91.53% respectively.While the positive rate of anti CCP or RF alone and both of patients without RA was 4.16%,29.16%,and 0 respectively.Through the analysis of statistics,the combined examination as well as the single examination great significance statistically.The positive rate of anti-CCP and RF of patients with RA was significantly higher than that of non-RA patients(χ2= 41.15、25.60 ;P0.01).Conclusion Determination of the combination of serum anti CCP and RF is helpful in the diagnosis of patients with RA.