Optimization of Plant Regeneration System in Vitro Culture of Creeping Dichondra with the Uniform Design

The optimization of plant regeneration system in vitro culture of creeping dichondra(Dichondra repens Forst.) was studied with the uniform design.The results indicated that the differentiation capability of callus from different induction medium were different,and the optimum medium supplemented with growth regulators for callus induction of creeping dichondra was MS+100 mg·L-1 casein enzymatic hydrolysate+0.5 mg·L-1 α-NAA + 0.2 mg·L-1 ZT.The differentiation capability of different explants were different.The differentiation rate of cotyledon was up to 60% in differentiation medium containing MS + 5.0 mg·L-1 6-BA+ 2.0 mg·L-1 ZT,and that of the hypocotyls was only to 5% in differentiation medium containing MS + 3.0 mg·L-1 6-BA + 3.0 mg·L-1 ZT + 0.3 mg·L-1 α-NAA.